![]() Career statistics Club As of match played 8 November 2020 Appearances and goals by club, season and competition In May 2018, he was named in Costa Rica’s 23 man squad for the 2018 World Cup in Russia. also Francisco played Argentina in a 3–0 loss. He made his debut in the competition in a game against Colombia, ended in a 0–1 loss, and a 2–0 win against Bolivia. He was also a reserve call-up for the Costa Rica team at the 2011 CONCACAF Gold Cup, and later impressed senior head coach Ricardo La Volpe to win him a call-up for the Costa Rica squad at the 2011 Copa América. He was also part of Costa Rica in the FIFA U-20 World Cup Colombia 2011. On he made his debut with the Costa Rica senior team in San José in a friendly game against Nigeria. This performance led to him being named in the MLS Team of the Week. On 5 March 2022, Calvo scored two late set piece goals for a ten-man San Jose team to salvage a 3-3 draw at home against the Columbus Crew. On 26 January 2022, Calvo signed as a free agent with San Jose Earthquakes. Following the 2021 season, Calvo's contract option was declined by Chicago. In 2018, Calvo was selected as an MLS All-Star, becoming the first Minnesota United player in club history to receive the honor. On 27 December 2016, Calvo joined Major League Soccer side Minnesota United FC. The following season, he continued to be a set-piece threat as he scored 9 goals in 24 matches playing as a defender. In his first season with Saprissa, he appeared in 39 matches and scored five goals. In January 2015, Calvo joined Santos de Guápiles as a loan. ![]() In January 2013 he moved abroad to play for then Danish champions Nordsjælland but he returned to Herediano in summer 2013. On his return to Costa Rica in 2011, Calvo played for Herediano before a stint at Pérez Zeledón where he scored 5 goals in 21 matches. The combination of pheromone attraction and nectar feeding is likely to be a generalized pollination syndrome in Pleurothallidinae.After starting with Deportivo Saprissa as a youth player from 2005 to 2010, Calvo moved to the United States to study at San Jacinto College after getting a scholarship and played football at university level during that period. It is not expected that Specklinia species will hybridize naturally as their populations do not overlap geographically. There seem to be no species-specific relationships between the orchids and the flies. ![]() endotrachys group share a similar pollination syndrome. Several different Drosophila species can be found on a single Specklinia species. The flies frequently show courtship behaviour, occasionally copulating. Visible nectar drops on the adaxial surface of sepals are secreted by nectar-secreting stomata, encouraging male and female Drosophila to linger on the flowers for several hours at a time. The flies are arrested by aggregation pheromones, including ethyl tiglate, methyl tiglate and isopropyl tiglate, released by the flowers, and to which at least D. ![]() spectabilis are visited and pollinated by several different but closely related Drosophila species. Floral compounds were analysed by gas chromatography–mass spectometry using those same pheromones as standards. Electroantennogram experiments were carried out on Drosophila hydei using the known aggregation pheromones ethyl tiglate, methyl tiglate and isopropyl tiglate. Tissue samples of the sepals, petals and labellum of Specklinia species were observed and documented by SEM, LM and TEM. Flies were photographed, filmed and observed for several days during a 2-year period and were identified by a combination of non-invasive DNA barcoding and anatomical surveys. Methods Specimens of Specklinia and Drosophila were collected in the field in Costa Rica and preserved in the JBL and L herbaria. endotrachys complex are pollinated by different Drosophila species. This study documents and describes how species of the S. Chase would later identify the visiting flies as being members of the genus Drosophila. Background and Aims The first documented observation of pollination in Pleurothallidinae was that of Endre´s, who noticed that the ‘viscid sepals’ of Specklinia endotrachys were visited by a ‘small fly’.
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